![]() ![]() Furthermore, no differences between animal category (P = 0.73) and FSH treatment (P = 0.53) were observed regarding the total follicles aspirated. There was no interaction between FSH treatment and animal category for all analyzed variables. Data were analyzed by the GLIMMIX procedure of SAS 9.4 ®. The same semen was used across all replicates and donor category. On Day 7, the progesterone device was removed and the OPU procedure was performed in both groups. Buffalo donors in the Control group received no further treatment, whereas buffalo donors in the FSH group received a total dosage of 200 mg im of FSH on Days 4 and 5, in four decreasing doses 12 h apart (57, 57, 43 and 43 mg). ![]() Buffaloes from different categories were then randomly allocated to one of two groups (Control or FSH), in a cross-over experimental design. ![]() A total of 54 buffalo donors (18 NU, 15 PR and 21MU) received an intravaginal progesterone device (1.0 g) plus estradiol benzoate at random stage of the estrous cycle (Day 0) during the breeding season (autumn and winter). The aim of this study was to evaluate the ovarian follicular population, the oocyte yield and the in vitro embryo production (IVEP) of nulliparous (NU), primiparous (PR) and multiparous (MU) buffalo donors submitted to the superstimulation with FSH prior to the ovum pick-up (OPU). ![]()
0 Comments
Leave a Reply. |